miR-103 Regulates Oxidative Stress by Targeting the BCL2/Adenovirus E1B 19 kDa Interacting Protein 3 in HUVECs

Figure 5

Cell viability and DCF analysis of HUVECs exposed to H₂O₂ with or without BNIP3 knockdown. (a) Western blot analysis showing levels of BNIP3 when HUVECs were transfected with different BNIP3 siRNA constructs or a negative control (NC) siRNA. (b) The CCK-8 assay was performed to evaluate the cell viability of HUVECs treated as indicated. (c) An apoptosis assay was used to assess the apoptosis levels of HUVECs treated as indicated. (d) Intracellular formation of ROS was measured in HUVECs treated as indicated. (e) BNIP3 expression was determined in HUVECs transfected with siRNA and treated with or without H₂O₂. , versus control and versus H₂O₂ group.

(a)

(b)

(c)

(d)

(e)