Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 9

Effect of in vitro replicative aging on SA-β-Gal expression in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). (a) Representative microphotographs of X-Gal staining in T21F and CF at low and high passages. (b) Fold increase in positive cell counts for X-Gal from low to high passages in T21F and CF. Cells were seeded in 12-well plates, stained with X-Gal. A minimum of 5 random fields were photographed ×100 with phase contrast for X-Gal-positive cell count. Results are expressed as mean ± SEM. Statistical differences between the two passage groups were analyzed by Student’s -test: .

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