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Oxidative Medicine and Cellular Longevity
Volume 2015 (2015), Article ID 536456, 10 pages
Research Article

Acute Exercise Induced Mitochondrial H2O2 Production in Mouse Skeletal Muscle: Association with p66Shc and FOXO3a Signaling and Antioxidant Enzymes

1School of Physical Education and Sports Science, Hangzhou Normal University, Hangzhou 311121, China
2National Institute of Complementary Medicine, University of Western Sydney, Penrith, NSW 2751, Australia
3Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai 200241, China
4College of Physical Education and Health, East China Normal University, Shanghai 200241, China

Received 6 August 2014; Revised 21 November 2014; Accepted 20 December 2014

Academic Editor: Y. Zhang

Copyright © 2015 Ping Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Exercise induced skeletal muscle phenotype change involves a complex interplay between signaling pathways and downstream regulators. This study aims to investigate the effect of acute exercise on mitochondrial H2O2 production and its association with , FOXO3a, and antioxidant enzymes. Male ICR/CD-1 mice were subjected to an acute exercise. Muscle tissues (gastrocnemius and quadriceps femoris) were taken after exercise to measure mitochondrial H2O2 content, expression of and FOXO3a, and the activity of antioxidant enzymes. The results showed that acute exercise significantly increased mitochondrial H2O2 content and expressions of and FOXO3a in a time-dependent manner, with a linear correlation between the increase in H2O2 content and or FOXO3a expression. The activity of mitochondrial catalase was slightly reduced in the 90 min exercise group, but it was significantly higher in groups with 120 and 150 min exercise compared to that of 90 min exercise group. The activity of SOD was not significantly affected. The results indicate that acute exercise increases mitochondrial H2O2 production in the skeletal muscle, which is associated with the upregulation of and FOXO3a. The association of and FOXO3a signaling with exercise induced H2O2 generation may play a role in regulating cellular oxidative stress during acute exercise.