Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway

Figure 1

Fragmentation of GA and apoptosis in N2a cells without transfection after OGDR. The experiment was repeated independently for at least three times. (a) Immunofluorescent stain using an antibody against Golgi marker Golgi58 (red color) and counterstain with 4′,6′-diamidino-2-phenylindole (blue color) to show nuclei revealed normal GA in normal conditions and after 4 h of OGD treatment. However, more and more N2a cells showed fragmented GA after 4 and 12 h reperfusion following 4 h OGD. (b) Quantitation (mean ± SEM) of (a) from three independent experiments. The increase of N2a cells with fragmented GA begun as early as 4 h reperfusion following 4 h OGD exposure and further enhances after 12 h reperfusion. (c) Apoptosis of N2a cells after OGDR insult. No significant difference was found between control and 0 h reperfusion following 4 h OGD. However, the proportion of apoptotic cells was significantly increased after 4 and 12 h reperfusion. compared to normal conditions (Bar = 10 μm).

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