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Figure 1: Protective effect of EDA against IAA-induced cells damage in CGNs. (a) Gross morphological change of CGNs cell body and neuritic network was determined by fluorescent immunostaining with antibody against βIII-tubulin (400x magnification). CGNs were pretreated with or without EDA for 2 h and then incubated with 50 μM IAA for another 4 h. (b) IAA-induced decrease in cell viability by MTT assay. CGNs were exposed to 25, 50, and 100 μM of IAA for 4 h. Cell viability was measured by MTT reduction assay. (c) EDA pretreatment attenuates IAA-induced neuronal loss in a concentration-dependent manner. CGNs were pretreated with or without EDA for 2 h and then incubated with 50 μM IAA for another 4 h. Cell viability was measured by MTT reduction assay. (d) Effect of EDA pretreatment on IAA-induced LDH release. Cells were treated as in (c) and LDH release level was detected by cytotoxicity detection kit. (e) Cotreatment of EDA with IAA inhibits cell viability decrease induced by IAA. CGNs were cotreated with EDA and IAA for 4 h. Cell viability was measured by MTT reduction assay. # versus control (Ctrl); *; ** and *** versus IAA alone group.