Sulfur Dioxide Enhances Endogenous Hydrogen Sulfide Accumulation and Alleviates Oxidative Stress Induced by Aluminum Stress in Germinating Wheat Seeds

Figure 6

Effect of SO₂ donor pretreatment on relative gene expression of TaWali1, TaWali2, TaWali3, TaWali5, TaWali6, and TaALMT1 in wheat radicals exposed to Al stress. Initially, wheat grains were geminated in water for 36 h. Then four treatment groups were done as follows, control, 60 h in H₂O; Pre-S, pretreatment with 1 mM SO₂ donor for 12 h, and then exposed to H₂O for 48 h; Al, 12 h in H₂O prior to exposure to 400 μM AlCl₃ for 48 h; Pre-S + Al, 12 h in 1 mM SO₂ donor pretreatment followed by 400 μM AlCl₃ for 48 h.