PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

Figure 1

H₂O₂ increases PRMT1 and PRMT4 expression but decreases SIRT1 expression. (a, b) ARPE-19 cells were treated with 250 μM H₂O₂ for 12 and 24 h. (a) Cell viability was measured by the MTT assay. The data represent the means ± SEM of three independent experiments, each performed in triplicate. versus 0 h. (b) Cell extracts were subjected to Western blotting with the indicated antibodies. The data represent the means ± SEM of three independent experiments. versus 0 h.

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