Research Article

hCLOCK Causes Rho-Kinase-Mediated Endothelial Dysfunction and NF-κB-Mediated Inflammatory Responses

Figure 4

Induction of the NK-κB pathway by RhoA. (a) Western blot analysis of RhoA pathway effector ROCK1 and NF-κB pathway effectors COX-2, IL-6, and p-P65 in HUVECs overexpressing hCLOCK and exposed to 5 μg/mL RhoA inhibitor CT-04 or control solution for 24 hours. Vector control group consists of HUVECs transduced with control retroviral vector exposed to control solution. (b) Bar graph showing relative ROS levels in the same HUVEC groups. compared to vector control.