Hydrogen Sulfide Donor GYY4137 Protects against Myocardial Fibrosis

Figure 5

Effect of GYY4137 on Ang II-induced collagen synthesis in cardiac fibroblasts. Neonatal rat cardiac fibroblasts were pretreated with different concentrations of GYY4137 for 4 h followed by Ang II (10⁻⁷ M) stimulation for an additional 24 h. (a-b) Quantification of collagen I and collagen III mRNA expression in myocardium was carried out with real-time RCR. (c-d) Cell lysates were tested for collagen I and collagen III protein expression by western blotting. Tubulin was probed as a loading control. (e) Cellular collagen I and III were visualized using Alexa Fluor 555 or Alexa Fluor 488 conjugated IgG, respectively, by immunofluorescence staining. The nuclei were counterstained with DAPI (scale bar: 100 μm). Plots represent mean ± SEM from 4–9 independent experiments. Cells treated with culture medium served as vehicle control (con). Statistical significance: , compared with con. group; , compared with Ang II stimulation alone.

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