TY - JOUR A2 - Quiles, José L. AU - Pan, Xiaoqi AU - Zhu, Lanlan AU - Lu, Huiping AU - Wang, Dun AU - Lu, Qing AU - Yan, Hong PY - 2015 DA - 2015/06/21 TI - Melatonin Attenuates Oxidative Damage Induced by Acrylamide In Vitro and In Vivo SP - 703709 VL - 2015 AB - Acrylamide (ACR) has been classified as a neurotoxic agent in animals and humans. Melatonin (MT) has been shown to be potentially effective in preventing oxidative stress related neurodegenerative disorders. In this study, whether MT exerted a protective effect against ACR-induced oxidative damage was investigated. Results in cells showed that reactive oxygen species (ROS) and malondialdehyde (MDA) significantly increased after ACR treatment for 24 h. MT preconditioning or cotreatment with ACR reduced ROS and MDA products, whereas the inhibitory effect of MT on oxidant generation was attenuated by blocking the MT receptor. Increased DNA fragmentation caused by ACR was significantly decreased by MT coadministration. In vivo, rats at 40 mg/kg/day ACR by gavage for 12 days showed weight loss and gait abnormality, Purkinje cell nuclear condensation, and DNA damage in rat cerebellum. MT (i.p) cotreatment with ACR not only recovered weight and gait of rats, but also decreased nuclear condensation and DNA damage in rat cerebellum. Using MDA generation, glutathione (GSH) level, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in rat cerebellum as indicators, MT alleviated ACR-induced lipid peroxidation and depressed antioxidant capacity. Our results suggest thatMT effectively prevents oxidative damage induced by ACR. SN - 1942-0900 UR - https://doi.org/10.1155/2015/703709 DO - 10.1155/2015/703709 JF - Oxidative Medicine and Cellular Longevity PB - Hindawi Publishing Corporation KW - ER -