Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells

Figure 4

Effects of Lico A on the Keap1/Nrf2/ARE signaling pathway in RAW264.7 cells. (a) Cells were treated with different concentration of Lico A (1.85, 3.7 and 7.4 μM) for 18 h, and the total protein were measured by Western blot analysis. (c) Cells were treated with Lico A (3.7 μM) indicated time periods, and the nuclear and cytoplasmic levels of Nrf2 were examined by Western blot analysis. (b and d) The relative density of protein was performed by densitometric analysis; β-actin and Lamin B acted as an internal control, respectively. (e) The luciferase plasmids pGL-ARE and pRL-TK was transiently transfected into cells for 24 h and subsequently exposed to 3.7 μM Lico A for the indicated periods. ARE luciferase activity was detected by a dual-luciferase reporter assay system. All results were expressed as means ± SEM of three independent experiments. , versus the control group.

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