Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells

Figure 6

Effects of Lico A on the activation of the PI3K/Akt and MAPK pathways in RAW 264.7 cells. Cells were treated with increasing doses of Lico A (1.85, 3.7 and 7.4 μM) for 18 h, and whole cell lysates were prepared and detected by Western blot analysis for phosphorylated and total Akt, ERK, JNK, and p38 protein expression. (b, d, f, and h) Quantification of induction of PI3K/Akt and MAPKs phosphorylation were performed by densitometric analysis and its unphosphorylated forms acted as an internal control, respectively. All results were expressed as means ± SEM of three independent experiments. , versus the control group.

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