Nrf2 Signaling and the Slowed Aging Phenotype: Evidence from Long-Lived Models

Figure 1

Nrf2 regulated protein expression in cardiac muscle from lifelong calorically restricted mice. Nrf2 was not different compared to ad libitum (AL) mice, while NQO1 and SOD-1 were significantly higher in cardiac muscle from CR mice compared to AL. Nrf2, NQO1, and SOD-1 were analyzed by western blotting and normalized to tubulin, shown below the proteins from each blot. Data are expressed as a ratio of target protein to tubulin (mean ± SEM). males in each condition. Lifelong B6D2F1 CR mice were maintained at the NIA colony at 40% food restriction compared with AL. Male mice were purchased at 6 months of age and divided into AL and CR groups. CR animals were maintained on NIH-31/NIA Fortified Diet, whereas AL animals were maintained on NIH-31 diet.