Comparative Meta-Analysis of Transcriptomics Data during Cellular Senescence and In Vivo Tissue Ageing

Figure 6

Significantly enriched KEGG pathways, which are deregulated during human ageing. (a) Venn diagram of KEGG pathways identified as overrepresented among the transcripts significantly up- or downregulated ( value ≤ 0.05; FDR ≤ 0.1) in cell- and biopsy-based transcriptomics; only KEGG pathways with count-threshold ≥2 (minimum number of genes for each KEGG pathway) were considered. The green circle represents the total number of KEGG pathways as derived by the combination of five different experiments, whereas the blue circle represents the number of KEGG pathways as derived by the analysis of seven different experiments; the overlapping area represents the common KEGG pathways (indicated in bold in Figure 6(b)). (b) Heatmap of the enrichment (%) score of predicted KEGG pathways in the context of the five cell- and seven biopsy-based datasets. Columns represent the enrichment score (%) of KEGG pathways per dataset and rows indicate the individual KEGG pathway; empty cells denote statistically not significant or no overrepresented KEGG pathway. M: male; F: female; skeletal muscle 1 (M): GDS287; skeletal muscle 2 (M): GDS156; skeletal muscle 3 (M): GDS288; skeletal muscle 4 (F): GDS472; skeletal muscle 5 (F): GDS473; skeletal muscle 6 (M, F): GSE5086; HSCs-1: PBCD34⁺38⁻ cells; HSCs-2: BMCD34⁺ cells.

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