Downregulation of Endogenous Hydrogen Sulfide Pathway Is Involved in Mitochondrion-Related Endothelial Cell Apoptosis Induced by High Salt

Figure 6

Apoptosis and caspase-3 activities in human umbilical vein endothelial cells (HUVECs). (a) Caspase-3 activities in HUVEC detected by western blot; (b) DNA fragmentation in HUVEC detected by Cell Death Detection ELISA. (c) HUVEC apoptosis detected by in situ Cell Death Detection Kit, Fluorescein. Control: the cell treated with 137 mmol/L sodium. HS: high salt, the cell treated with 200 mmol/L sodium for 6 h. HS + NaHS: the cell pretreated with 200 μmol/L NaHS for 30 min following 200 mmol/L sodium for 6 h. versus control group; versus HS group.

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