Genome-Wide Expression Profiling of Anoxia/Reoxygenation in Rat Cardiomyocytes Uncovers the Role of Mito in Energy Homeostasis

Figure 1

Illustration of the experimental A/R model protocols. Cardiomyocytes were cultured for 20 hours in normoxia incubator. Petri dishes were randomly distributed to 4 groups. Cardiomyocytes of Con were continuously cultured in normoxia environment for 105 min. Medium of A/R group was replaced with N₂ bubbled (95% N₂, 5% CO₂) modified M199 at the 40th min and then replaced with O₂ bubbled modified M199 at 45th and 50th min. Medium of DZ group was replaced with N₂ bubbled modified M199 at the 40th min; at 45th min, medium was replaced with O₂ bubbled modified M199 containing 50 μM DZ and at the 50th min it was replaced with O₂ bubbled modified M199 to remove DZ. Medium of DZ5HD group was replaced with N₂ bubbled modified M199 at 40th min containing 100 μM 5-HD; at 45th min, it was replaced with O₂ bubbled modified M199 containing 50 μM DZ and then replaced with O₂ bubbled modified M199 to remove DZ at 50th min.