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Oxidative Medicine and Cellular Longevity
Volume 2015 (2015), Article ID 758358, 14 pages
http://dx.doi.org/10.1155/2015/758358
Research Article

Cardiac H2S Generation Is Reduced in Ageing Diabetic Mice

1Shanghai Key Laboratory of Bioactive Small Molecules and Research Center on Aging and Medicine, Department of Physiology and Pathophysiology, Fudan University Shanghai Medical College, Shanghai 200032, China
2Department of Physiology, Hebei Medical University, Hebei 050017, China
3Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China

Received 22 October 2014; Revised 5 January 2015; Accepted 5 January 2015

Academic Editor: Guangdong Yang

Copyright © 2015 Sheng Jin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. To examine whether hydrogen sulfide (H2S) generation changed in ageing diabetic mouse hearts. Results. Compared to mice that were fed tap water only, mice that were fed 30% fructose solution for 15 months exhibited typical characteristics of a severe diabetic phenotype with cardiac hypertrophy, fibrosis, and dysfunction. H2S levels in plasma, heart tissues, and urine were significantly reduced in these mice as compared to those in controls. The expression of the H2S-generating enzymes, cystathionine γ-lyase and 3-mercaptopyruvate sulfurtransferase, was significantly decreased in the hearts of fructose-fed mice, whereas cystathionine-β-synthase levels were significantly increased. Conclusion. Our results suggest that this ageing diabetic mouse model developed diabetic cardiomyopathy and that H2S levels were reduced in the diabetic heart due to alterations in three H2S-producing enzymes, which may be involved in the pathogenesis of diabetic cardiomyopathy.