Cardiac H₂S Generation Is Reduced in Ageing Diabetic Mice

Figure 6

Reduction of endogenous H₂S involves in high glucose-induced myocardial injury. (a) Neonatal rat cardiac ventricular myocytes (NRCMs) viability measured by CCK-8 assay at the end of the treatment for 72 h. (b) Representative images of cardiomyocyte apoptosis detected by a laser confocal microscope at the end of the treatment for 72 h. (c) Quantitative analysis for cardiomyocyte apoptosis determined by flow cytometry. (d) Representative images of ROS levels in NRCMs detected by a laser confocal microscope at the end of the treatment for 72 h. (e) Quantitative analysis for ROS levels in NRCMs. (f) H₂S levels in NRCMs at the end of the treatment for 72 h. NG group, normal glucose (5.5 mmol/L); HG group, high glucose (33 mmol/L); Osmotic pressure control group, normal glucose (5.5 mmol/L) + L-glucose (27.5 mmol/L). Results are means ± SEM. A of <0.05 was considered significant.

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