Oxidative Medicine and Cellular Longevity / 2015 / Article / Fig 7

Research Article

Cardiac H2S Generation Is Reduced in Ageing Diabetic Mice

Figure 7

Exogenous H2S attenuates high glucose-induced myocardial injury. (a) Exogenous NaHS caused transient increase of H2S level in cell culture media within 6 hours. versus HG group; versus HG group ( in each group). (b) H2S levels in neonatal rat cardiac ventricular myocytes (NRCMs) at the end of the treatment for 72 h. (c) NRCMs viability measured by CCK-8 assay at the end of the treatment for 72 h. (d) Representative images of cardiomyocyte apoptosis detected by a laser confocal microscope at the end of the treatment for 72 h. (e) Quantitative analysis for cardiomyocyte apoptosis determined by flow cytometry. (f) Representative images of ROS levels in NRCMs detected by a laser confocal microscope at the end of the treatment for 72 h. (g) Quantitative analysis for ROS levels in NRCMs. NG group, normal glucose (5.5 mmol/L); HG group, high glucose (33 mmol/L). Results are means ± SEM. A of <0.05 was considered significant.
(a)
(b)
(c)
(d)
(e)
(f)
(g)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19.