Research Article

Effect of Myricetin, Pyrogallol, and Phloroglucinol on Yeast Resistance to Oxidative Stress

Figure 2

Effect of myricetin, pyrogallol, and phloroglucinol on intracellular oxidation and oxidative damage. Yeast cells were grown in YPD medium to the exponential phase and pretreated with compounds (300 μM) or equal volume of DMSO (control) for 15 min and subsequently treated with 1.5 mM H2O2 for 1 h. (a) Representative histograms of intracellular ROS analyzed by flow cytometry using H2DCF-DA as a probe. (b) Quantification of intracellular ROS expressed by mean fluorescence intensity in 10,000 cells (arbitrary units) from at least 3 independent assays. (c) Quantitative analysis of protein carbonyl content was performed by densitometry using data taken from the same membrane. Proteins were derivatized with DNPH and slot-blotted into a PVDF membrane. Immunodetection was performed using an anti-DNP antibody. Values are mean ± SEM of at least 3 independent assays. Values were compared by two-way ANOVA, Sidak’s multiple comparisons test ().