The Neuroprotective Effects of Ratanasampil on Oxidative Stress-Mediated Neuronal Damage in Human Neuronal SH-SY5Y Cells

Figure 1

The effects of RNSP on H₂O₂-induced toxicity in SH-SY5Y cells. (a) The cell viability of SH-SY5Y cells after treatment with RNSP for 5 days. (b) The cell viability of SH-SY5Y cells after 24 h of exposure to H₂O₂. (c) The effect of RNSP on H₂O₂-induced toxicity in SH-SY5Y cells. Pretreatment was performed with RNSP (5 days) before exposure to H₂O₂ (100 μM, 24 h). Each column and bar represent the mean ± SEM ( each). Asterisks indicate a statistically significant difference from the value in untreated cells (, ). Pound signs indicate a statistically significant difference from the value in H₂O₂-exposed cells without pretreatment with RNSP (^#, ^##). (d) The morphological changes of SH-SY5Y cells with or without pretreatment with RNSP (60 μg/mL, 5 days) after exposure to H₂O₂ for 24 h. Scale bar = 20 μm.

(a)

(b)

(c)

(d)