A Nitric Oxide-Donor Furoxan Moiety Improves the Efficacy of Edaravone against Early Renal Dysfunction and Injury Evoked by Ischemia/Reperfusion

Figure 6

Effects of EDV and NO-EDV on iNOS expression and p65 NF-κB nuclear translocation. Representative Western blot and corresponding densitometric analysis of the bands showing iNOS protein expression (panel (a)) and NF-κB p65 subunit translocation from the cytosol to the nucleus (panel (b)) were evaluated in the kidney homogenates of rats subjected to the surgical procedure alone (sham) or rats subjected to I/R treated with vehicle or EDV (6 and 30 μmol/kg, i.v.) or NO-EDV (1.2 and 6 μmol/kg, i.v.). Each immunoblot is from a single experiment and is representative of three separate experiments. Densitometric analysis of the related bands is expressed as relative OD, corrected for the corresponding β-actin contents, and normalized using the related sham-operated band. The data from bands densitometric analysis are mean S.E.M. versus vehicle.

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