Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKK/NF-κB-Dependent Mechanism

Figure 3

AOPP-increased MCP-1 expression in podocytes was mainly mediated by the IKK/NF-κB pathway. The overnight serum-deprived podocytes were treated with 200 μg/mL AOPPs for 30 min. The expression of p-IKKα/β, p-NF-κB p65, and IκBα was examined by Western blotting (a, b, and c). To verify the role of the IKK/NF-κB pathway involved in the AOPP-induced MCP-1 expression, protein levels of MCP-1 were determined by ELISA using cell supernatants exposed to AOPPs for 24 h in the presence of the IKK/NF-κB inhibitor PTL (d). Data are expressed as the means ± SEM of three independent experiments. ANOVA, (a, b, and c) , versus CON. (d) versus CON, versus AOPP. CON, untreated cells; MSA, mouse serum albumin.

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