Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKK/NF-κB-Dependent Mechanism

Figure 5

SLs decreased the AOPP-induced expression of MCP-1 mRNA in a dose- and time-dependent manner. Podocytes were preincubated with the indicated concentration of MCL, compound 1, or compound 2 for 1 h before treatment with 200 μg/mL of AOPPs for 24 h. PTL (5 μM) was used as a positive control (a, b, and c). Aliquots of 200 μg/mL AOPPs were used to treat podocytes for 24 h, and then MCL (5 μM), compound 1 (10 μM), or compound 1 (2.5 μM) was added for the indicated times (d, e, and f). The expression levels of MCP-1 mRNA were determined by qPCR. SLs decreased the AOPP-induced expression of MCP-1 mRNA in a dose- and time-dependent manner. The data are expressed as the means ± SEM of three independent experiments. ANOVA, versus CON; , , and versus AOPP. CON, untreated cells; MSA, mouse serum albumin.

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