Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKK/NF-κB-Dependent Mechanism

Figure 6

MCL prevented AOPP-induced IKKβ and NF-κB p65 activation and IκBα degradation in a dose- and time-dependent manner in podocytes. After 24 h of serum starvation, podocytes were pretreated with MCL (2.5 μM, 5 μM, and 10 μM) for 1 h and then exposed to 200 μg/mL AOPPs or native MSA for 30 min. PTL (10 μM) was used as a positive control (a, b, and c). Alternatively, podocytes were treated with 200 μg/mL AOPPs for 30 min, and then MCL (10 μM) was added for a preset time (d, e, and f). The protein expression of p-IKKα/β, p-NF-κB p65, and IκBα was analyzed by Western blotting. The data are expressed as the means ± SEM of three independent experiments. ANOVA, , versus CON; , , and versus AOPP. CON, untreated cells; MSA, mouse serum albumin.

(a)

(b)

(c)

(d)

(e)

(f)