Effects of Synthetic Serum Supplementation in Sperm Preparation Media on Sperm Capacitation and Function Test Results

Figure 3

Tyrosine phosphorylation levels of protein of 105 kDa according to western blotting (a), acrosome reaction rates (b), DNA fragmentation according to the TUNEL test (c), and oxidative injury according to the 8-OHdG test (d) in spermatozoa cultured in various sperm preparation media. and denote and , respectively, compared with spermatozoa in the HTS capacitating condition according to the Wilcoxon signed-rank test. The arrow in (b) indicates a spermatozoon with a reacted acrosome. The black circles denote all data points that lie outside the 10th and 90th percentiles.

(a)

(b)

(c)

(d)