The Nutraceutical Dehydrozingerone and Its Dimer Counteract Inflammation- and Oxidative Stress-Induced Dysfunction of In Vitro Cultured Human Endothelial Cells: A Novel Perspective for the Prevention and Therapy of Atherosclerosis

Figure 6

ICAM-1 and VCAM-1 gene expression. ICAM-1 (a) and VCAM-1 (b) expression analysis by quantitative real-time PCR (qRT-PCR). HUVEC were cultured in endothelial cell basal medium containing 10 μg/mL of DHZ or DHZ dimer or medium alone. After an overnight incubation, cells were stimulated with 10 ng/mL of human recombinant TNF-α or medium alone for 6, 24, and 48 hours. Total cellular RNA was extracted at each time point and mRNA levels were analyzed. Error bars represent SD. (a) ; ; ; ; . (b) ; ; ; ; .

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