(a)
(b)
Figure 4: Oral administration of ME resulted in decreased protein oxidative damage in RBCs and whole blood. The RBCs and whole blood of the subjects were collected at 0, 1, 4, 12, 16, and 24 weeks after beginning oral administration of ME. (a) A representative blot shows HNE staining using a western blotting technique to determine the protein oxidative damage in RBCs and whole blood. Actin was used as a loading control. (b) Quantitative analysis of the extent of HNE modification to RBCs (HNE-RBC) and whole blood (HNE-WB) after oral administration of ME was carried out as that relative to week 0. Data are presented as means (). Error bars represent SEM. The asterisk indicates statistical significance: in comparison with week 0 (-test).