Research Article

Inhibition of Receptor Interacting Protein Kinases Attenuates Cardiomyocyte Hypertrophy Induced by Palmitic Acid

Figure 4

AKT/mTOR mediates necroptosis in the PA-induced cardiomyocyte hypertrophy. (a) Pretreating the NCMs with rapamycin (1 μM), the mRNA level of ANP and BNP was decreased, . (b) Pretreating the NCMs with Nec-1 (10 nM), the upregulated Ser473 of AKT and Ser2481 of mTOR phosphorylation by PA stimulation (western blot) was inhibited. (c) Quantitative analysis of western blots (b), . (d) Pretreating the NCMs with rapamycin (1 μM) had no effect on the upregulated RIPK1 by PA stimulation via western blot, . Data in (a), (c), and (d) are expressed as mean ± SD, indicates compared to control treatment, and indicates compared to PA treatment.
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