Carvacrol Alleviates Prostate Cancer Cell Proliferation, Migration, and Invasion through Regulation of PI3K/Akt and MAPK Signaling Pathways

Figure 1

Carvacrol (CAR) inhibited TRPM7-like currents in PCa cells. (a) TRPM7 protein expression detected by western blotting ( versus RWPE-1 cells, ). (b) PC-3 and DU145 cells were treated with carvacrol (500 μM) for 24 h. TRPM7 protein expression was detected by western blot. (c) Representative current traces of inward and outward currents at +100 mV and −100 mV (). The current traces were started to record when the TRPM7-like currents reached a platform after the finish of the whole cell configuration. Both inward and outward currents were inhibited by carvacrol (500 μM), and they recovered after carvacrol washout. (d) Representative current-voltage trace of TRPM7-like current in PC-3 cells treated with either vehicle control (pretreated) or carvacrol (500 μM). (e) Statistical analysis of current density at +100 mV and −100 mV in PC-3 cells ( versus pretreated, ). (f) Representative current-voltage trace of TRPM7-like current in DU145 cells treated with either vehicle control or carvacrol (500 μM). (g) Statistical analysis of current density at +100 mV and −100 mV in DU145 cells ( versus pretreated, ).

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