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Figure 2: Carvacrol reduced PCa cell proliferation. (a) PC-3 and DU145 cells were treated with 125~1000 μM for 24 h, then CCK-8 assay was conducted, and IC50 was calculated through nonlinear regression fitting (). IC50 in PC-3 and DU145 cells were μM and μM, respectively. OD is the abbreviation of optical density. (b) PC-3 cells (the left panel) and DU145 cells (the right panel) were treated with either vehicle control or carvacrol with 250, 500, and 750 μM for 24, 48, and 72 hours, respectively. Then cell proliferation curves were detected by CCK-8 assay ( versus control, ). (c) Carvacrol reduced colony formation of PC-3 cells (, ). (d) Carvacrol reduced colony formation of DU145 cells (, ). refers to the comparisons of carvacrol at 250 and 500 μM, respectively.