Carvacrol Alleviates Prostate Cancer Cell Proliferation, Migration, and Invasion through Regulation of PI3K/Akt and MAPK Signaling Pathways

Figure 6

TRPM7 knockdown regulates PC-3 functions. (a) TRPM7 protein expression was determined by western blot after PC-3 infected with lentivirus vector (sh-Control and sh-TRPM7) for 72 h ( versus sh-Control, ). (b) After 72 h infection with either sh-Control or sh-TRPM7 lentivirus, TRPM7-like currents were recorded using patch clamp. Representative I-V traces of PC-3 cells were shown (). (c) Statistical analysis of patch clamp results was shown. TRPM7 knockdown significantly reduced the currents at +100 mV and −100 mV (, ). (d) TRPM7 knockdown significantly inhibited PC-3 cell proliferation detected by CCK-8 assay ( versus sh-Control, ). (e) TRPM7 knockdown significantly inhibited PC-3 cell migration detected by wound healing assay. The representative images were shown (). (f) Statistical analysis of wound healing (, ). (g) TRPM7 knockdown significantly inhibited PC-3 cell invasion detected by Transwell assay ( versus sh-Control, ). (h) TRPM7 knockdown significantly reduced p-Akt and p-ERK1/2 protein levels in PC-3 cells ( versus sh-Control, ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)