Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos

Figure 3

Dysfunction of mitochondria in mouse zygotes induced by H₂O₂. (a) Representative images of MMP in mouse zygotes at 6 h from treated group and control group; red fluorescence from channel 1 represented J-aggregates (high polarized mitochondria); green fluorescence from channel 2 represented monomer form of JC-1 (low polarized mitochondria). (b) The analysis of MMP was via the comparisons of relative fluorescence intensity and set the average value of red/green fluorescence intensity at each time point from control group as 100%; MMP in treated group was showed relative to control group at the corresponding point time. MMP in treated zygotes declined during the first hour after treatment by 0.03 mM H₂O₂ for 30 min and kept on dropping over the subsequent hours. Data are presented as mean ± SD in three independent experiments. , the treated group versus the control group (Student’s -test).

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