Differential Regulation of the Extracellular Cysteine/Cystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice
Expression levels of the CySS transporter subunit Slc7a11 and the fibronectin EDA splice variant in young and old fibroblasts. Lung fibroblasts from young and old mice were plated in 6-well plates at a density of 800,000 cells per well in complete DMEM. Cells were collected 24 hours later, and RNA was isolated for RT-PCR analysis. (a) Decreased expression of Slc7a11 in lung fibroblasts harvested from aged animals when compared to those obtained from young animals. (b) Increased expression of fibronectin EDA in lung fibroblasts harvested from aged animals when compared to those obtained from young animals ().