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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 1578235, 8 pages
http://dx.doi.org/10.1155/2016/1578235
Research Article

Impact of Oxidative Stress in Premature Aging and Iron Overload in Hemodialysis Patients

1Unidad de Investigación en Epidemiología Clínica, Servicio de Hemodiálisis, Unidad Médica de Alta Especialidad (UMAE) No. 1 Bajío, Instituto Mexicano del Seguro Social (IMSS), León, GTO, Mexico
2Departamento de Investigaciones Médicas, Universidad de Guanajuato, León, GTO, Mexico
3Unidad de Investigación Médica en Enfermedades Oncológicas, CMN, SXXI, IMSS, 06720 Ciudad de México, Mexico

Received 1 April 2016; Revised 15 July 2016; Accepted 23 August 2016

Academic Editor: Claudio Cabello-Verrugio

Copyright © 2016 Blanca Murillo-Ortiz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Increased oxidative stress is a well described feature of patients in hemodialysis. Their need for multiple blood transfusions and supplemental iron causes a significant iron overload that has recently been associated with increased oxidation of polyunsaturated lipids and accelerated aging due to DNA damage caused by telomere shortening. Methods. A total of 70 patients were evaluated concomitantly, 35 volunteers with ferritin levels below 500 ng/mL (Group A) and 35 volunteers with ferritin levels higher than 500 ng/mL (Group B). A sample of venous blood was taken to extract DNA from leukocytes and to measure relative telomere length by real-time PCR. Results. Patients in Group B had significantly higher plasma TBARS (), carbonyls (), and urea () compared with those in Group A. Telomeres were significantly shorter in Group B, 0.66 (SD, 0.051), compared with 0.75 (SD, 0.155) in Group A (). We observed a statistically significant association between relative telomere length and ferritin levels (, ). Relative telomere length was inversely related to time on hemodialysis (, ). Conclusions. Our findings demonstrate that iron overload was associated with increased levels of oxidative stress and shorter relative telomere length.