Research Article
Functional Interactions between BKCaα-Subunit and Annexin A5: Implications in Apoptosis
Figure 3
α subunit colocalizes with ANXA5. (a) Reciprocal coimmunoprecipitation from lysates of HEK 293 cells stably expressing α subunit with anti-α antibody (+). Bead controls (−) consisted of lysate mixed with DynaG beads lacking antibody. (b) Reciprocal coimmunoprecipitation from lysates of HEK 293 cells stably expressing α subunit with and anti-ANXA5 antibody (+) and bead control (−). (c) HEK 293 cells stably expressing α subunit were immunostained with anti-α subunit (1 : 250, red) and anti-ANXA5 (1 : 200 green) antibodies and cell nuclei (blue) where visualized with DAPI. Scale bars are 50 μM.
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