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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 1687657, 12 pages
http://dx.doi.org/10.1155/2016/1687657
Research Article

Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm

1Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Avenue Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508 270 Sao Paulo, SP, Brazil
2Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain

Received 25 September 2015; Revised 30 November 2015; Accepted 2 December 2015

Academic Editor: Paola Venditti

Copyright © 2016 Thais Rose dos Santos Hamilton et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material contains tables describing mean, median, standard errors, quartiles and p value of different variables of ejaculated sperm such as motility, sperm concentration, mass motility, total defects, mayor defects, minor defects, sperm thiobarbituric acid reactive substances, seminal plasma thiobarbituric acid reactive substances, stressed cells, high mitochondrial membrane potential, low mitochondrial membrane potential, intermediate mitochondrial membrane potential, percentage of sperm cells with intact membrane and intact acrosome, percentage of sperm cells with membrane and damaged acrosome, percentage of sperm cells with damaged membrane and intact acrosome, percentage of sperm cells with damaged membrane and damaged acrosome, glutathione peroxidase enzymatic activity, glutathione reductase enzymatic activity, superoxide dismutase enzymatic activity, catalase enzymatic activity, immunodetection of catalase, immunodetection of superoxide dismutase, immunodetection of glutathione reductase, immunodetection of glutathione peroxidase considering the treatment effect between treated and control groups in rams submitted or not to heat stress.

  1. Supplementary Material