Berberine Attenuates Myocardial Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation Response: Role of Silent Information Regulator 1

Figure 10

Effects of BBR and SIRT1 siRNA on oxidative stress and inflammation response in SIR-injured cardiomyocyte. H9C2 cardiomyocytes were exposed to control siRNA or SIRT1 siRNA and then treated with BBR (50 μmol/L for 8 hr). Next, cells were subjected to simulated ischemia/reperfusion (SIR, 2 hr/4 hr) treatment. Then, oxidative stress level was measured. (a) Cardiomyocyte superoxide generation. (b) expression. Top images: representative blots. (c) Cell supernatants IL-6 level. (d) Cell supernatants TNF-α level. The results are expressed as the mean ± SEM, /group. versus control siRNA group, versus SIR + control siRNA group, and versus SIR + BBR + control siRNA group.

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