Berberine Attenuates Myocardial Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation Response: Role of Silent Information Regulator 1

Figure 8

Effects of berberine and sirtinol treatment on SIRT1, Ac-Foxo1, caspase-3, Bcl-2, and Bax expression in MI/R-injured heart. Normal SD rats were exposed to BBR treatment (200 mg/kg/d for 2 weeks) in the absence or presence of sirtinol (Stnl, 2 mg/kg/d for 1 week) and then subjected to myocardial ischemia reperfusion (MI/R) operation. SIRT1-related signaling and apoptosis-related protein were measured after 4 hr of reperfusion. (a) Representative blots. (b) SIRT1 expression. (c) Ac-Foxo1 expression. (d) Caspase-3 expression. (e) Bcl-2 expression. (f) Bax expression. The results are expressed as the mean ± SEM, /group. versus Sham group, versus MI/R + V group, and versus MI/R + BBR group.

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