Research Article

Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line

Figure 3

Amla treatment stimulated antioxidant systems by Nrf2 activation. (a) Activation of Nrf2 was analyzed using an ARE luciferase assay. Data are expressed as relative activities (reporter luciferase activity/control luciferase activity) as compared with data from control cells. ; . (b and c) C2C12 myotubes were incubated with Amla (200 μg/mL) for 48 h. (b) Nuclear lysates were analyzed by western blot, and YY-1 was used an internal control for nuclear protein. ; . (c) Relative levels of mRNA for antioxidant system related genes were analyzed by RT-qPCR. ; ; . 18S rRNA was used as an internal control for RT-qPCR.