Oxidative Medicine and Cellular Longevity / 2016 / Article / Tab 1 / Research Article
Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line Table 1 Primer sets used for RT-qPCR.
Gene name F/R Sequence (5′ to 3′) Internal standard 18S rRNA F TTCCGATAACGAACGAGACTCT R TGGCTGAACGCCACTTGTC Mitochondrial biogenesis PGC1α F CCAAACCCACAGAAAACAGG PPAR-gamma coactivator 1-alpha R TGGGGTCATTTGGTGACTCT NRF1 F GAACTGCCAACCACAGTCAC Nuclear respiratory factor 1 R TCGTCTGGATGGTCATTTCA mtTFA F CCGAAGTGTTTTTCCAGCAT Mitochondrial transcriptional factor A R GGCTGCAATTTTCCTAACCA Antioxidant system Nrf2 F GGGAGAAAACGACAGAAACC Nuclear factor erythroid 2 related factor 2 R TGGGAGAGTAAGGCTTTCCA HO-1 F TGACACCTGAGGTCAAGCAC Heme oxygenase-1 R TCCTCTGTCAGCATCACCTG NQO1 F AAACGTCTGGAAACCGTCTG Nadph dehydrogenase quinone 1 R TTCTGCTCCTCTTGAACTTCC Cat F GGACGCTCAGCTTTTCATTC Catalase R TTGTCCAGAAGAGCCTGGAT GPx F CTCATGACCGACCCCAAGTA Glutathione peroxidase R CCCACCAGGAACTTCTCAAA Mn-SOD F TCTGTGGGAGTCCAAGGTTC Manganese superoxide dismutase R TAAGGCCTGTTGTTCCTTGC Cu-SOD F GAGACCTGGGCAATGTGACT Copper superoxide dismutase R TCATGGACCACCATTGTACG
