Low-ω3 Fatty Acid and Soy Protein Attenuate Alcohol-Induced Fatty Liver and Injury by Regulating the Opposing Lipid Oxidation and Lipogenic Signaling Pathways

Figure 4

Influence of EtOH, low-ω3FA, and SP on lipogenic pathway. Total RNA from each animal was reverse transcribed and used in the qRT-PCR analysis using gene specific primers as described in Section 2 for (a) PGC1β, (b) SREBP1c, (c) ACC, and (d) c-Met. Each gene was normalized to β-actin mRNA. Nuclear or total protein was extracted from each animal and used for Western Blot analysis using specific antibodies as described in Section 2 for (e) PGC1β, (f) SREBP1c, (g) ACC, and (h) c-Met. Values are means of triplicate experiments ± SD of 3 samples/group and were corrected for difference in loading after reprobing with an antibody to Lamin B1 or β-actin for nuclear or total protein, respectively. versus control; versus EtOH.

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