Tissue Kallikrein Alleviates Cerebral Ischemia-Reperfusion Injury by Activating the B2R-ERK1/2-CREB-Bcl-2 Signaling Pathway in Diabetic Rats

Figure 5

(A) FJC staining was performed 24 h after MCAO to assess neuron degeneration. (B) The number of FJC-positive cells was counted 24 h after MCAO. (C) TUNEL staining was performed 24 h after MCAO to assess apoptosis in neurons. (D) The number of TUNEL-positive cells was counted 24 h after MCAO. (E) Cleaved caspase-3 staining was performed 24 h after MCAO to assess neuron apoptosis. (F) The number of cleaved caspase-3-positive cells was counted after MCAO. (a) Sham; (b) saline; (c) TK; (d) B1RA+TK; (e) B2RA+TK; (f) B1RA+B2RA+TK; (g) DMSO; (h) U0126; (i) U0126+TK. Scale bar = 100 mm. Data are expressed as mean ± SD; rats/group. Six representative microscopic fields were analyzed for each rat ( versus sham group; versus saline group; versus TK group; versus B1RA+TK group; versus B2RA+TK group).