Hypochlorite-Modified Albumin Upregulates ICAM-1 Expression via a MAPK–NF-κB Signaling Cascade: Protective Effects of Apocynin

Figure 7

Effects of DPI and SOD on signal transduction underlying HOCl-alb-induced ICAM-1 expression, ROS generation, and phosphorylation of ERK1/2 and in HUVECs. ((a) and (b)) HUVECs were preincubated with the indicated concentrations of DPI (a) or SOD (b) for 1 h and stimulated with 200 mg/L HOCl-alb for 12 h. ICAM-1 protein expression was analyzed with western blotting. ((c), (d), and (e)) HUVECs were preincubated with apocynin, DPI, or SOD for 1 h and then stimulated with 200 mg/L HOCl-alb. Total intracellular ROS was measured with DCFDA fluorescence intensity (c), superoxide in cell homogenates was detected by Lucigenin-enhanced chemiluminescence assay (d), and the activation of ERK1/2 and were determined with western blotting (e). Data are expressed as the means ± SD of three independent experiments. ANOVA, in (a), (b), (c), (d) and (e). versus control; versus the HOCl-alb group.

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