C1q/TNF-Related Protein 9 Protects Diabetic Rat Heart against Ischemia Reperfusion Injury: Role of Endoplasmic Reticulum Stress

Figure 4

DsbA-L knockdown increased ERS in H9c2 cells and attenuated CTRP9 induced protection against SIR injury. Both scramble and DsbA-L suppressed cells were subjected to SIR injury at the presence of globular CTRP9 (1 μg/mL). Representative images (a) and bar graphs of TNF-α (b), Caspase-9 (c), Caspase-3 (d), Caspase-12 (i), GRP-78 (e), CHOP (f), and DsbA-L (g) in H9c2 cardiomyocytes were determined by Western blots. GAPDH was used as loading control. Representative images (h) and bar graph (j) of apoptotic cardiomyocytes were shown. The apoptotic cells were detected by immunofluorescent staining with TUNEL ((h), upper panel), and DAPI staining was used to label the nuclei ((h), lower panel). The results were expressed as the mean ± SEM, per group. compared with scramble.

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