Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

Figure 4

RES treatment of STZ-induced diabetes reduces AKI sensitivity and oxidative stress level. After I/R 48 hours, kidney tissues were collected for H&E staining and the histological damage score (a, c) and TUNEL assay of apoptosis (b, d) were examined; blood samples were used to measure BUN (e), serum creatinine (f) levels, and kidney tissues for analysis of superoxide anion radical scavenging capacity (g), MDA production (h), and SOD content (i). The data in (c–i) are means ± SE (). versus DS group; versus DI/R group. NS and DS: nondiabetic and STZ-induced diabetic rats were subjected to sham operation. NI/R and DI/R: nondiabetic and STZ-induced diabetic rats were subjected to 25 min ischemia followed by 48 h reperfusion. DI/R-RES: STZ-induced diabetic rats that underwent I/R were treated with RES (10 mg/kg, ip daily) for 7 consecutive days before renal ischemia-reperfusion.

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