Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

Figure 5

STZ-induced diabetes induces TXNIP expression and NLRP3 inflammasome activation following I/R 48. TXNIP expression was examined by IHC (a), representative blots (b), and quantitative analysis of Western blots for TXNIP (c), NLRP3 (d) and pro-caspase-1 and cleaved caspase-1 (e-f), and release of IL-1 and IL-18 by ELISA (g-h). The data in (c–h) are means ± SE (). versus NS group; versus DS group; versus NI/R group; versus DI/R group. NS and DS: nondiabetic and STZ-induced diabetic rats were subjected to sham operation. NI/R and DI/R: nondiabetic and STZ-induced diabetic rats were subjected to 25 min ischemia followed by 48 h reperfusion. DI/R-RES: STZ-induced diabetic rats that underwent I/R were treated with RES (10 mg/kg, ip daily) for 7 consecutive days before renal ischemia-reperfusion.

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