Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

Figure 7

Western blot analysis of TXNIP and NLRP3 protein expression in cultured HK-2 cells treated by normal glucose (5.5 mM), high glucose (30 mM), and NG + mannitol, respectively, for 72 hours, then following 4 hours of hypoxia and 2 hours of reoxygenation in HK-2 cells under high glucose stimulation with or without TXNIP siRNA and RES treatment, respectively. Representative blots (a) and quantitative analysis of Western blots for TXNIP (c) and NLRP3 (d), activity of caspase-1 (e), level of IL-1β (f), and Western blot of TXNIP gene knockdown in HK-2 cells (b). The data in (c–f) are means ± SE (). versus NG group; ^☆ versus NH/R group; versus HH/R-scrambled siRNA group.

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