Research Article

Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

Figure 9

Effect of TXNIP inhibition on the HH/R-induced apoptosis and oxidative stress in HK-2 cells. Apoptotic cells were defined as the cells in the right two quadrants of each plot and the percentages were determined by flow cytometry (a, b); intracellular ROS was detected by flow cytometry (c); SOD (d) and MDA (e) were detected by microplate reader. The data in (b–e) are means ± SE (). versus NG group; versus NH/R group; versus HH/R-scrambled siRNA group. NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG condition; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG condition. HH/R-RES: HH/R pretreated by RES (50 μM) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R.
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