Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity

Figure 4

Influence of amine oxidase substrates or inhibitors and phenolic compounds on fluorescent-based detection of hydrogen peroxide. The chromogenic mixture used for Amplex Red-based fluorometric detection of hydrogen peroxide was incubated with 5 μM H₂O₂ alone (control set at 100%) or in the presence of the indicated final concentrations of tested chemicals. (a) Lack of influence of MAO and SSAO substrates or inhibitors. (b) Dose-dependent abolishment by phenolic compounds of the fluorescent signal elicited by 5 μM hydrogen peroxide. Means ± SEM of 7 measurements without any human material.

(a)

(b)